Course Description: Biochemistry 551 is an integrated lecture, lab and seminar course that covers biochemistry-centered theory and techniques. The course is designed for upper-level undergraduate students majoring in Biochemistry. Students learn how to apply a broad range of biochemical, genetic, and physical techniques to modern biochemical research. Students also learn how to analyze and interpret the primary scientific literature, develop an understanding of the communication of data, and connect biochemical techniques to basic research.

Lectures introduce concepts and theory that are subsequently explored in detail in experiments. The virtual labs are designed to provide experience with techniques that are used in modern biochemical research through interactive online activities. The curriculum incorporates a research project beginning with the PCR amplification and cloning of the HCAII gene, which codes for the enzyme human carbonic anhydrase II (HCAII). As the semester progresses, students explore how to overexpress, purify and assay wild type and mutant HCAII protein. Experiments covered include PCR, spectrophotometry, gel electrophoresis, protein overexpression and purification, enzyme assays and fluorescence spectroscopy. Several times during the semester, at-home lab experiments are incorporated to provide hands-on experience to supplement student understanding of the virtual labs.

This lab manual contains detailed descriptions of each online laboratory exercise in this course. Please see the course Canvas site for other course information, such as: a syllabus, schedule, assignment guidelines, and lecture and seminar materials.

Table of Contents:

Lab 1: Structural analysis of HCAII using PyMOL
Lab 2: PCR Amplification of HCAII and pETblue2
Lab 3: Lab-at-home: Introduction to Biotechnology Methods
Lab 4: Gibson assembly of HCAII into pETblue2 vector and transformation of E. coli with the reactions
Lab 5: Screening for pETblue2-HCAII clones
Lab 6: Mutant Exploration
Lab 7: Protein Expression and Purification in E. coli
Lab 8: Analysis of His-tagged HCAII Expression and Purification
Lab 9: Determination of Protein Stability using Chemical Denaturation and Intrinsic Fluorescence
Lab 10: Investigation of wild type and mutant HCAII enzyme activity
Lab 11: Forster Resonance Energy Transfer (FRET) to detect ligand-binding to HCAII

We are happy to welcome you to our second Open Educational Resource (OER) textbook, Biochemistry Free For All. Biochemistry is a relatively young science, but its rate of growth has been truly impressive. The rapid pace of discoveries, which shows no sign of slowing, is reflected in the steady increase in the size of biochemistry textbooks. Growing faster than the size of biochemistry books have been the skyrocketing costs of higher education and the even faster rising costs of college textbooks. These unfortunate realities have created a situation where the costs of going to college are beyond the means of increasing numbers of students.

Table of Contents
Basic Biology
Basic Chemistry
Water and Buffers
Amino Acids
Protein Structure
Structure and Function of Nucleic Acids
Structure and Function of Carbohydrates
Structure and Function of Lipids
Membranes: Basic Concepts
Membranes: Transport
Membranes: Other Considerations
Catalysis: Basic Principles
Catalysis: Control of Activity
Catalysis: Mechanisms
Blood Clotting
Energy: Basics
Electron Transport and Oxidative Phosphorylation
Photophosphorylation
Metabolism of Sugars
Metabolism of Polysaccharides
Citric Acid Cycle
Metabolism of Fats and Fatty Acids
Metabolism of Other Lipids
Metabolis of Amino Acids and the Urea Cycle
Metabolism of Nucleotides
Genes and Genomes
DNA Replication
DNA Repair
Transcription
RNA Processing
Translation
Regulation of Gene Expression
Cell Signaling
Basic Techniques
Point by Point: In the Beginning
Point by Point: Structure and Function
Point by Point: Membranes
Point by Point: Catalysis
Point by Point: Energy
Point by Point: Metabolism
Point by Point: Information Processing
Point by Point: Techniques

We are happy to welcome you to our second Open Educational Resource (OER) textbook, Biochemistry Free For All. Biochemistry is a relatively young science, but its rate of growth has been truly impressive. The rapid pace of discoveries, which shows no sign of slowing, is reflected in the steady increase in the size of biochemistry textbooks. Growing faster than the size of biochemistry books have been the skyrocketing costs of higher education and the even faster rising costs of college textbooks. These unfortunate realities have created a situation where the costs of going to college are beyond the means of increasing numbers of students.

Our goal is to present the key observations and unifying concepts upon which modern biology is based; it is not a survey of all biology! Once understood, these foundational observations and concepts should enable you to approach any biological process, from disease to kindness, from a scientific perspective. To understand biological systems we need to consider them from two complementary perspectives; how they came to be (the historic, that is, evolutionary) and how their structures, traits, and behaviors are produced (the mechanistic, that is, the physicochemical)

Our goal is to present the key observations and unifying concepts upon which modern biology is based; it is not a survey of all biology! Once understood, these foundational observations and concepts should enable you to approach any biological process, from disease to kindness, from a scientific perspective. To understand biological systems we need to consider them from two complementary perspectives; how they came to be (the historic, that is, evolutionary) and how their structures, traits, and behaviors are produced (the mechanistic, that is, the physicochemical).

Table of Contents
Chapter 1: Understanding science & thinking scientifically
Chapter 2: Life's diversity and origins
Chapter 3: Evolutionary mechanisms and the diversity of life
Chapter 4: Social evolution and sexual selection
Chapter 5: Molecular interactions, thermodynamics & reaction coupling
Chapter 6: Membrane boundaries and capturing energy
Chapter 7: The molecular nature of heredity
Chapter 8: Peptide bonds, polypeptides and proteins
Chapter 9: Genomes, genes, and regulatory networks
Chapter 10: Social systems

Biological anthropology, also known as physical anthropology, is a scientific discipline concerned with the biological and behavioral aspects of human beings, their related non-human primates and their extinct hominin ancestors. It is a subfield of anthropology that provides a biological perspective to the systematic study of human beings. This textbook explores evolutionary theory, including the core concepts of basic genetics and the modern synthesis of evolution. Students will examine, critically evaluate and explain scientific claims about the origins of humankind and modern human variation as well as biocultural evolution. Students will develop critical thinking and communication skills through the application of essential anthropological approaches, theories, and methods.

Table of Contents:
I: Evolutionary Theory
II: Non-Human Primates
III: Human Evolution

Biology 2e is designed to cover the scope and sequence requirements of a typical two-semester biology course for science majors. The text provides comprehensive coverage of foundational research and core biology concepts through an evolutionary lens. Biology includes rich features that engage students in scientific inquiry, highlight careers in the biological sciences, and offer everyday applications. The book also includes various types of practice and homework questions that help students understand—and apply—key concepts.

The 2nd edition has been revised to incorporate clearer, more current, and more dynamic explanations, while maintaining the same organization as the first edition. Art and illustrations have been substantially improved, and the textbook features additional assessments and related resources.

Table of Contents
Preface

1. The Chemistry of Life

2. The Cell

3. Genetics

4. Evolutionary Processes

5. Biological Diversity

6. Plant Structure and Function

7. Animal Structure and Function

8. Ecology

The Periodic Table of Elements

Geological Time

Measurements and the Metric System

Biology 2e is designed to cover the scope and sequence requirements of a typical two-semester biology course for science majors. The text provides comprehensive coverage of foundational research and core biology concepts through an evolutionary lens. Biology includes rich features that engage students in scientific inquiry, highlight careers in the biological sciences, and offer everyday applications. The book also includes various types of practice and homework questions that help students understand—and apply—key concepts.

I. The Study of Life
II. The Chemical Foundation of Life
III. Biological Macromolecules
IV. Cell Structure
V. Structure and Function of Plasma Membranes
VI. Metabolism
VII. Cellular Respiration
VIII. Photosynthesis
IX. Cell Communication
X. Cell Reproduction
XI. Meiosis and Sexual Reproduction
XII. Mendel's Experiments and Heredity
XIII. Modern Understandings of Inheritance
XIV. DNA Structure and Function
XV. Genes and Proteins
XVI. Gene Expression
XVII. Ethics and Societal Responsibility

A systematic study of the structure, function, ecology and evolution or organisms including bacteria, protists, fungi, plants and animals.
Chapter I. Evolution and the Origin of Species
Chapter II. The Evolution of Populations
Chapter III. Viruses
Chapter IV. Prokaryotes: Bacteria and Archaea
Chapter V. Protists
Chapter VI. Fungi
Chapter VII. Introduction to Animal Diversity
Chapter VIII. Invertebrates
Chapter IX. Vertebrates
Chapter X. Plant Form and Physiology
Chapter XI. Plant Reproduction
Chapter XII. The Animal Body: Basic Form and Function
Chapter XIII. Animal Nutrition and the Digestive System
Chapter XIV. The Nervous System
Chapter XV. Sensory Systems
Chapter XVI. The Endocrine System
Chapter XVII. The Musculoskeletal System
Chapter XVIII. The Respiratory System
Chapter XIX. The Circulatory System
Chapter XX. Osmotic Regulation and Excretion
Chapter XXI. The Immune System
Chapter XXII. Animal Reproduction and Development
Chapter XXIII. Ecology and the Biosphere
Chapter XXIV. Population and Community Ecology
Chapter XXV. Ecosystems
Chapter XXVI. Conservation Biology and Biodiversity

An introduction to biology intended for non-science majors.  Focus areas include chemical foundations, cell structure and division, genetics, and evolution.

This template course was developed from generally available open educational resources (OER) in use at multiple institutions, drawing mostly from a primary work published by OpenStax College Concepts of Biology, but also including additional open works from various sources as noted in attributions on each page of materials.

Includes the study of the gross and microscopic structure of the systems of the human body with special emphasis on the relationship between structure and function. Integrates anatomy and physiology of cells, tissues, organs, the systems of the human body, and mechanisms responsible for homeostasis.

Also available here:https://courses.lumenlearning.com/atd-herkimer-biologyofaging/

Table of Contents:

I. Chapter 1: Introduction to Human Aging
II. Chapter 2: Theories of Aging
III. Chapter 3: Cellular Aging
IV. Chapter 4: The Integumentary System
V. Chapter 5: Bone Tissue and The Skeletal System
VI. Chapter 6: The Skeletal Muscle System
VII. Chapter 7: The Nervous System
VIII. Chapter 8: The Special Senses
IX. Chapter 9: The Circulatory System
X. Chapter 10: The Immune System
XI. Chapter 11: The Respiratory System
XII. Chapter 12: The Digestive System
XIII. Chapter 13: The Urinary System
XIV. Chapter 14: The Reproductive System
XV. Chapter 15: The Endocrine System
XVI. Course Information
XVII. Term Research Project

This is a custom textbook catered to the needs of kinesiology students enrolled in a first-year biomechanics course. It has been modified from OpenStax College Physics and Anatomy and Physiology.

I. Chapter 1: Prerequisite Skills for Biomechanics
II. Chapter 2: Anatomy Basics
III. Chapter 3: Linear Kinematics in One-dimension
IV. Chapter 4: Linear Kinematics in Two-dimensions
V. Chapter 5: Angular Kinematics
VI. Chapter 6: Linear Kinetics
VII. Chapter 7: Work, Power, and Energy
VIII. Chapter 8: Angular Kinetics
IX. Chapter 9: Mechanics of Human Tissues
X. Chapter 10: Mechanism of Injury

Health technology innovation in low- and middle-income countries (LMICs), including countries in Africa, falls far short of meeting the healthcare needs of these settings. The result is a heavy reliance on products and technologies imported from industrialised countries that are often not suited to, or sustainable for, LMICs.

Appropriate healthcare products for LMICs are best developed in these countries, where local knowledge and understanding of needs, context and available resources may be incorporated into designs and implementation plans. The objectives for enabling health technology development in LMICs include: 1) expanding the base of expertise through research training programmes with a problem-solving focus; 2) stimulating new knowledge, approaches and solutions by enabling innovation; and 3) integrating research communities within and across institutions to build critical mass.

The field of biomedical engineering is central to health technology innovation. This book is a response to the need for biomedical engineering capacity in Africa. It is grounded in the African context. It serves as a resource for academics and students in biomedical engineering, for those interested in entering the field in any capacity and for practitioners at every stage of product development. University leaders intent on establishing new biomedical engineering programmes or departments, may draw on the content for guidance on structuring their offerings. The book reaches beyond Africa, as it is relevant to other LMIC settings, and provides insights to guide global health initiatives focused on technology innovation.

Given the broad definition of biotechnology applications and products, it is easy to see how there is enormous overlap within the fields of cellular biology, microbiology, chemistry, and biomedical engineering. It is the goal of this textbook to provide foundational knowledge to begin building your biotechnology toolkit and enter an exciting career of making a difference through biotechnology.

This textbook first explores the fundamentals of laboratory science and biotechnology. Unit 1 begins with a dive into the foundation of biology and chemistry by asking what is life and what are the molecules of life? The end of the unit offers a primer into laboratory science; preparing solutions and operating basic lab equipment. Unit 2 brings readers through a vital tool of biomanufacturing, understanding the growth and control of microbes. Many biotechnology products are made by harnessing the work of microbes, and this unit explores this invisible and intriguing world of microorganisms. The final unit, molecular biotechnology, offers a more in-depth look at how biological molecules such as DNA and protein are manipulated into creating useful products.

Table of Contents
1 Unit 1. The Foundations of Biotechnology

Chapter 1. The Study of Life
Chapter 2. The Building Blocks of Life
Chapter 3. What is Biotechnology?
Chapter 4. Biotechnician Tools: Measurements & Uncertainty
Chapter 5. Biotechnician Tools: Preparing Solutions
Chapter 6. Biotechnician Tools: Basic Laboratory Equipment
2 Unit 2. Introduction to Biomanufacturing

Chapter 7. Cell Structure and Function
Chapter 8. Microbes
Chapter 9. Microbial Techniques
Chapter 10. Microbial Growth
Chapter 11. Control of Microbial Growth
3 Unit 3. Molecular Biotechnology

Chapter 12. Nucleic Acid Structure & Function
Chapter 13. Protein Structure and Function
Chapter 14. Laboratory Techniques: Nucleic Acids and Proteins
Chapter 15. Viruses, Vaccines, and the Immune System
Chapter 16. Immunochemistry

La Biotecnología Ambiental emplea el uso de organismos para la prevención, monitoreo y remediación de los ecosistemas, al mismo tiempo permite la generación de bioproductos y energía limpia. El libro consistió en tres capítulos, en el primero se establece los fundamentos de la Biotecnología Ambiental y sus aplicaciones en entornos terrestre, acuático y aéreo; además, se presentan herramientas aplicadas como los biosensores en el monitoreo ambiental, la fitorremediación en varios contextos de América Latina y el fortalecimiento de la agricultura sustentable. En el segundo capítulo se describe la importancia de un estudio bibliométrico y el enfoque para las diversas investigaciones, se detalla un manual de uso, que permite su replicabilidad en otros contextos. Además de presentar un caso de estudio aplicado al tema central de este libro, que sistematiza los temas más estudiados, las tendencias, revistas y países con mayores contribuciones. En el tercer capítulo se presentan varias prácticas de laboratorio y una sección de preguntas, que pueden ser empleadas en otras instituciones educativas o de manera autónoma. En conclusión, los capítulos son complementarios entre sí, facilitando la comprensión e interés del lector. El área de mayor relevancia corresponde a la biorremediación en suelos contaminados por hidrocarburos y metales pesados, los grupos más frecuentes en la revisión fueron hongos, cianobacterias y organismos genéticamente modificados. Finalmente, existe una oportunidad del uso y la potencial combinación de las técnicas basadas en metagenómica, ADN ambiental, inteligencia artificial para el desarrollo de las nuevas tendencias de la Biotecnología Ambiental

This book,"Blended Learning in K-12", is an overview of the concept of "blended learning" and how it relates to the integration of technologies in the K-12 environment. Dr. N.I. Scagnoli, the course instructor, has guided the students of the CTER 7 cohort in the creation of this book. This could not have happened in the 1980s. (By way of explanation to non-Americans, K-12 means all primary and secondary schooling).

Blender 3D: Noob to Pro is a product of shared effort by numerous team members and anonymous editors. Its purpose is to teach people how to create three-dimensional computer graphics using Blender, a free software application. This book is intended to be used in conjunction with other on-line resources that complement it.

A college students' guide for career success, this free Open Educational Resource text focuses on major identification and career exploration.

The Blueprint for Success series comprises three books for the College Success and FYE (First-Year Experience) genre. The central text, Blueprint for Success in College and Career (https://press.rebus.community/blueprint1/), is designed to show how to be successful in college and in career preparation. In addition, targeted sections on Study Skills and Time Management, and Career and Decision Making are available separately as Blueprint for Success in College: Indispensable Study Skills and Time Management Strategies (https://press.rebus.community/blueprint2/), and Blueprint for Success in Career Decision Making (https://press.rebus.community/blueprint3/).