This class analyzes complex biological processes from the molecular, cellular, extracellular, and organ levels of hierarchy. Emphasis is placed on the basic biochemical and biophysical principles that govern these processes. Examples of processes to be studied include chemotaxis, the fixation of nitrogen into organic biological molecules, growth factor and hormone mediated signaling cascades, and signaling cascades leading to cell death in response to DNA damage. In each case, the availability of a resource, or the presence of a stimulus, results in some biochemical pathways being turned on while others are turned off. The course examines the dynamic aspects of these processes and details how biochemical mechanistic themes impinge on molecular/cellular/tissue/organ-level functions. Chemical and quantitative views of the interplay of multiple pathways as biological networks are emphasized. Student work will culminate in the preparation of a unique grant application in an area of biological networks.

Each chapter in this book corresponds to a lab in the CHEM 3753: Introduction to Biochemical Methods course at the University of Oklahoma. All of the materials you will need for each lab can be found within its respective chapter. Each chapter will contain a brief introduction; a set of learning objectives; a slide presentation, screencast, or lab demonstration video; the protocol to be followed during your time in the lab; and a set of interactive quiz questions to help you check you understanding as you go. Other interactive features include photos from the lab, links to safety data sheets (SDS), and 3D chemical structures. Components of the book are elaborated upon below. We hope you find this book to be an all-in-one, fun, and engaging learning tool for the biochemical methods course.

" The course, which spans two thirds of a semester, provides students with a research-inspired laboratory experience that introduces standard biochemical techniques in the context of investigating a current and exciting research topic, acquired resistance to the cancer drug Gleevec. Techniques include protein expression, purification, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing. This class is part of the new laboratory curriculum in the MIT Department of Chemistry. Undergraduate Research-Inspired Experimental Chemistry Alternatives (URIECA) introduces students to cutting edge research topics in a modular format. Acknowledgments Development of this course was funded through an HHMI Professors grant to Professor Catherine L. Drennan."

Biology 2e is designed to cover the scope and sequence requirements of a typical two-semester biology course for science majors. The text provides comprehensive coverage of foundational research and core biology concepts through an evolutionary lens. Biology includes rich features that engage students in scientific inquiry, highlight careers in the biological sciences, and offer everyday applications. The book also includes various types of practice and homework questions that help students understand—and apply—key concepts. The 2nd edition has been revised to incorporate clearer, more current, and more dynamic explanations, while maintaining the same organization as the first edition. Art and illustrations have been substantially improved, and the textbook features additional assessments and related resources.

By the end of this section, you will be able to do the following:

Explain how the structure of DNA reveals the replication process
Describe the Meselson and Stahl experiments

By the end of this section, you will be able to do the following:

Discuss the different types of mutations in DNA
Explain DNA repair mechanisms

By the end of this section, you will be able to do the following:

Discuss the similarities and differences between DNA replication in eukaryotes and prokaryotes
State the role of telomerase in DNA replication

By the end of this section, you will be able to do the following:

Explain the process of DNA replication in prokaryotes
Discuss the role of different enzymes and proteins in supporting this process

By the end of this section, you will be able to do the following:

Describe the structure of DNA
Explain the Sanger method of DNA sequencing
Discuss the similarities and differences between eukaryotic and prokaryotic DNA

By the end of this section, you will be able to do the following:

Explain transformation of DNA
Describe the key experiments that helped identify that DNA is the genetic material
State and explain Chargaff’s rules

Biology is designed for multi-semester biology courses for science majors. It is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand. To meet the needs of today’s instructors and students, some content has been strategically condensed while maintaining the overall scope and coverage of traditional texts for this course. Instructors can customize the book, adapting it to the approach that works best in their classroom. Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand—and apply—key concepts.

By the end of this section, you will be able to do the following:

Describe the structure of prokaryotic and eukaryotic genomes
Distinguish between chromosomes, genes, and traits
Describe the mechanisms of chromosome compaction

By the end of this section, you will be able to do the following:

Describe nucleic acids' structure and define the two types of nucleic acids
Explain DNA's structure and role
Explain RNA's structure and roles